Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reverse-transcriptase polymerase chain reaction.
Identifieur interne : 000577 ( Main/Exploration ); précédent : 000576; suivant : 000578Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reverse-transcriptase polymerase chain reaction.
Auteurs : J A Weigl [Allemagne] ; W. Puppe ; B. Gröndahl ; H J SchmittSource :
- European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [ 0934-9723 ] ; 2000.
Descripteurs français
- KwdFr :
- Adolescent (MeSH), Allemagne (MeSH), Chlamydophila pneumoniae (classification), Chlamydophila pneumoniae (génétique), Chlamydophila pneumoniae (isolement et purification), Enfant (MeSH), Enfant d'âge préscolaire (MeSH), Hospitalisation (MeSH), Humains (MeSH), Infections de l'appareil respiratoire (microbiologie), Infections de l'appareil respiratoire (virologie), Infections de l'appareil respiratoire (épidémiologie), Infections à Chlamydophila (microbiologie), Infections à Chlamydophila (épidémiologie), Maladies virales (virologie), Mycoplasma pneumoniae (isolement et purification), Nourrisson (MeSH), Nouveau-né (MeSH), Partie nasale du pharynx (microbiologie), Partie nasale du pharynx (virologie), Pneumopathie à mycoplasmes (microbiologie), Pneumopathie à mycoplasmes (épidémiologie), RT-PCR (MeSH), Répartition par âge (MeSH), Saisons (MeSH), Virus (classification), Virus (génétique), Virus (isolement et purification).
- MESH :
- génétique : Chlamydophila pneumoniae, Virus.
- isolement et purification : Chlamydophila pneumoniae, Mycoplasma pneumoniae, Virus.
- microbiologie : Infections de l'appareil respiratoire, Infections à Chlamydophila, Partie nasale du pharynx, Pneumopathie à mycoplasmes.
- virologie : Infections de l'appareil respiratoire, Maladies virales, Partie nasale du pharynx.
- épidémiologie : Infections de l'appareil respiratoire, Infections à Chlamydophila, Pneumopathie à mycoplasmes.
- Adolescent, Allemagne, Chlamydophila pneumoniae, Enfant, Enfant d'âge préscolaire, Hospitalisation, Humains, Nourrisson, Nouveau-né, RT-PCR, Répartition par âge, Saisons, Virus.
- Wicri :
- geographic : Allemagne.
English descriptors
- KwdEn :
- Adolescent (MeSH), Age Distribution (MeSH), Child (MeSH), Child, Preschool (MeSH), Chlamydophila Infections (epidemiology), Chlamydophila Infections (microbiology), Chlamydophila pneumoniae (classification), Chlamydophila pneumoniae (genetics), Chlamydophila pneumoniae (isolation & purification), Germany (MeSH), Hospitalization (MeSH), Humans (MeSH), Infant (MeSH), Infant, Newborn (MeSH), Mycoplasma pneumoniae (isolation & purification), Nasopharynx (microbiology), Nasopharynx (virology), Pneumonia, Mycoplasma (epidemiology), Pneumonia, Mycoplasma (microbiology), Respiratory Tract Infections (epidemiology), Respiratory Tract Infections (microbiology), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Seasons (MeSH), Virus Diseases (virology), Viruses (classification), Viruses (genetics), Viruses (isolation & purification).
- MESH :
- geographic : Germany.
- classification : Chlamydophila pneumoniae, Viruses.
- epidemiology : Chlamydophila Infections, Pneumonia, Mycoplasma, Respiratory Tract Infections.
- genetics : Chlamydophila pneumoniae, Viruses.
- isolation & purification : Chlamydophila pneumoniae, Mycoplasma pneumoniae, Viruses.
- microbiology : Chlamydophila Infections, Nasopharynx, Pneumonia, Mycoplasma, Respiratory Tract Infections.
- virology : Nasopharynx, Respiratory Tract Infections, Virus Diseases.
- Adolescent, Age Distribution, Child, Child, Preschool, Hospitalization, Humans, Infant, Infant, Newborn, Reverse Transcriptase Polymerase Chain Reaction, Seasons.
Abstract
The aim of this study was to generate urgently needed data on respiratory pathogens in German children using an economical and efficient tool. Nasopharyngeal aspirates of hospitalized children 0-16 years of age with an acute respiratory tract infection were tested by a nine-valent multiplex reverse-transcriptase polymerase chain reaction. Of 1281 children, 449 (35%) had an acute respiratory tract infection caused by at least one of the organisms studied; there were 29 cases of dual infection. At least 34-42% of severe acute respiratory tract infections in children under 5 years of age were caused by viruses. In children over 5 years of age, this proportion was 23% (P<0.001). Infection during the first 2 years of life was most frequently due to respiratory syncytial virus (n = 162 cases). Parainfluenza virus type 3 (n = 22) and type 1 (n = 14) were detected almost exclusively in children under 5 years of age. Influenza A (n = 90) and adenoviruses (n = 98) were prevalent in all age groups. The frequency of influenza B virus isolation (n = 17) rose significantly after the age of 5 years. Mycoplasma pneumoniae infection (n = 24 cases, 5.2%) was most frequent in 5- to 16-year-old patients. Only one case of Chlamydia pneumoniae infection was found. Since the distribution of pathogens within the different types of lower respiratory tract infections is very similar, it seems that host factors determine which form of lower respiratory tract infection develops in an individual patient. The multiplex reverse-transcriptase polymerase chain reaction may, in the future, become an important tool for epidemiological studies as well as for individual diagnosis.
DOI: 10.1007/s100960050490
PubMed: 10898133
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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generate urgently needed data on respiratory pathogens in German children using an economical and efficient tool. Nasopharyngeal aspirates of hospitalized children 0-16 years of age with an acute respiratory tract infection were tested by a nine-valent multiplex reverse-transcriptase polymerase chain reaction. Of 1281 children, 449 (35%) had an acute respiratory tract infection caused by at least one of the organisms studied; there were 29 cases of dual infection. At least 34-42% of severe acute respiratory tract infections in children under 5 years of age were caused by viruses. In children over 5 years of age, this proportion was 23% (P<0.001). Infection during the first 2 years of life was most frequently due to respiratory syncytial virus (n = 162 cases). Parainfluenza virus type 3 (n = 22) and type 1 (n = 14) were detected almost exclusively in children under 5 years of age. Influenza A (n = 90) and adenoviruses (n = 98) were prevalent in all age groups. The frequency of influenza B virus isolation (n = 17) rose significantly after the age of 5 years. Mycoplasma pneumoniae infection (n = 24 cases, 5.2%) was most frequent in 5- to 16-year-old patients. Only one case of Chlamydia pneumoniae infection was found. Since the distribution of pathogens within the different types of lower respiratory tract infections is very similar, it seems that host factors determine which form of lower respiratory tract infection develops in an individual patient. The multiplex reverse-transcriptase polymerase chain reaction may, in the future, become an important tool for epidemiological studies as well as for individual diagnosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10898133</PMID><DateCompleted><Year>2000</Year><Month>11</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0934-9723</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>5</Issue><PubDate><Year>2000</Year><Month>May</Month></PubDate></JournalIssue><Title>European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</Title><ISOAbbreviation>Eur. J. Clin. Microbiol. Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Epidemiological investigation of nine respiratory pathogens in hospitalized children in Germany using multiplex reverse-transcriptase polymerase chain reaction.</ArticleTitle><Pagination><MedlinePgn>336-43</MedlinePgn></Pagination><Abstract><AbstractText>The aim of this study was to generate urgently needed data on respiratory pathogens in German children using an economical and efficient tool. Nasopharyngeal aspirates of hospitalized children 0-16 years of age with an acute respiratory tract infection were tested by a nine-valent multiplex reverse-transcriptase polymerase chain reaction. Of 1281 children, 449 (35%) had an acute respiratory tract infection caused by at least one of the organisms studied; there were 29 cases of dual infection. At least 34-42% of severe acute respiratory tract infections in children under 5 years of age were caused by viruses. In children over 5 years of age, this proportion was 23% (P<0.001). Infection during the first 2 years of life was most frequently due to respiratory syncytial virus (n = 162 cases). Parainfluenza virus type 3 (n = 22) and type 1 (n = 14) were detected almost exclusively in children under 5 years of age. Influenza A (n = 90) and adenoviruses (n = 98) were prevalent in all age groups. The frequency of influenza B virus isolation (n = 17) rose significantly after the age of 5 years. Mycoplasma pneumoniae infection (n = 24 cases, 5.2%) was most frequent in 5- to 16-year-old patients. Only one case of Chlamydia pneumoniae infection was found. Since the distribution of pathogens within the different types of lower respiratory tract infections is very similar, it seems that host factors determine which form of lower respiratory tract infection develops in an individual patient. The multiplex reverse-transcriptase polymerase chain reaction may, in the future, become an important tool for epidemiological studies as well as for individual diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Weigl</LastName><ForeName>J A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Pediatric Infectious Diseases, Department of General Pediatrics, Christian Albrechts University, Kiel, Germany. weigl@pediatrics.uni-kiel.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Puppe</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Gröndahl</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Schmitt</LastName><ForeName>H J</ForeName><Initials>HJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Eur J Clin Microbiol Infect Dis</MedlineTA><NlmUniqueID>8804297</NlmUniqueID><ISSNLinking>0934-9723</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000293" MajorTopicYN="N">Adolescent</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017677" MajorTopicYN="N">Age Distribution</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D023521" MajorTopicYN="N">Chlamydophila Infections</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016993" MajorTopicYN="N">Chlamydophila pneumoniae</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006760" MajorTopicYN="N">Hospitalization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007231" MajorTopicYN="N">Infant, Newborn</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009177" MajorTopicYN="N">Mycoplasma pneumoniae</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011019" MajorTopicYN="N">Pneumonia, Mycoplasma</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="Y">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012621" MajorTopicYN="N">Seasons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014777" MajorTopicYN="N">Virus Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014780" MajorTopicYN="N">Viruses</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>7</Month><Day>18</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>7</Month><Day>18</Day><Hour>11</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10898133</ArticleId><ArticleId IdType="doi">10.1007/s100960050490</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Schleswig-Holstein</li></region><settlement><li>Kiel</li></settlement></list><tree><noCountry><name sortKey="Grondahl, B" sort="Grondahl, B" uniqKey="Grondahl B" first="B" last="Gröndahl">B. Gröndahl</name><name sortKey="Puppe, W" sort="Puppe, W" uniqKey="Puppe W" first="W" last="Puppe">W. Puppe</name><name sortKey="Schmitt, H J" sort="Schmitt, H J" uniqKey="Schmitt H" first="H J" last="Schmitt">H J Schmitt</name></noCountry><country name="Allemagne"><region name="Schleswig-Holstein"><name sortKey="Weigl, J A" sort="Weigl, J A" uniqKey="Weigl J" first="J A" last="Weigl">J A Weigl</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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